The invention discloses a full-fabric microflow chip based on lateral opening and dumbbell optical fibers. The full-fabric microflow chip comprises lateral opening optical fibers 1, a dumbbell opticalfiber 2, a quartz sleeve 3, sampling sealing modules 4, a super-continuous spectrum light source 5, a micro-syringe pump 6, an effluent tank 7, a spectrometer 8 and a computer 9; two sections of lateral opening optical fibers are welded on the two sides of the dumbbell optical fiber; after the corrosion treatment, 50nm thick SPR (surface plasmon resonance) metallic membrane plating and sleeving of the quartz sleeve, two ducts of the left lateral opening optical fiber are respectively loaded with the sampling sealing modules and are connected with the micro-syringe pump, and the two ducts of the right lateral opening optical fiber are respectively loaded with the sampling sealing modules and are connected with the effluent tank; an output single mode fiber of the super-continuous spectrumlight source is welded with the left lateral opening optical fiber; the right lateral opening optical fiber is welded with the single mode fiber and then is connected with the spectrometer. Accordingto the microflow chip, an all-fiber SPR detecting microflow chip is constructed; the upper and lower ducts can realize time division multiplexing dual-channel detection; different metallic membranes can be plated on the upper and lower ducts so as to realize the dual-channel detection based on a wavelength-division multiplexing technology.