The invention belongs to the field of drug or health care product, and more specifically relates to a gynura formosana Kitam. total flavonoids extract, a preparation method thereof, and applications of the gynura formosana Kitam. total flavonoids extract in treating alcoholic fatty liver. The gynura formosana Kitam. total flavonoids extract comprises, by weight, 80 to 85% of rutin. Drug effect experiments are carried out taking zebra fish alcoholic fatty liver models as experimental models. It is shown by experiment results that the gynura formosana Kitam. total flavonoids extract possesses protective effect on alcoholic fatty liver, is capable of protecting livers, and can be taken as a potential drug used for treating alcoholic liver injury. According to the preparation method, after extraction, a composite enzyme is composed of enzymes of specific composition and specific ratio, and is used for enzymatic hydrolysis; and in addition, macroporous resin extraction condensation and macroporous resin separation purifying are adopted. The HPLC purity of rutin in the gynura formosana Kitam. total flavonoids extract is capable of reaching 80 to 85%.