The invention belongs to the field of drug or health care product, and more specifically relates to a gynura formosana Kitam. total flavonoids extract, a preparation method thereof, and applications of the gynura formosana Kitam. total flavonoids extract in treating hyperuricemia. The gynura formosana Kitam. total flavonoids extract comprises, by weight, 80 to 85% of rutin. It is shown by drug effect experiment results that the gynura formosana Kitam. total flavonoids extract is capable of reducing the activity of xanthine oxidase in the livers of hyperuricemia mouse models and inhibiting systhesis of uric acid at a certain degree, and reducing uric acid at a certain degree, and can be taken as a potential drug used for treating hyperuricemia or gout. According to the preparation method, after extraction, a composite enzyme is composed of enzymes of specific composition and specific ratio, and is used for enzymatic hydrolysis; and in addition, macroporous resin extraction condensationand macroporous resin separation purifying are adopted. The HPLC purity of rutin in the gynura formosana Kitam. total flavonoids extract is capable of reaching 80 to 85%.