The invention relates to a biosafety cabinet. The biosafety cabinet comprises a cabinet body, a cabinet door, a control unit and a storage unit, wherein two fingerprint recognition devices are arranged on the cabinet door, a plurality of safety grids are arranged in the cabinet body, a fingerprint recognition device is arranged on a safety door of each safety grid, further two door locks are arranged on the safety door of each safety grid, each safety grid is used for accommodating different protection levels and different kinds of biological materials, the control unit is used for controllingall components of the bisafety cabinet, and the storage unit is used for storing varieties of related data of the biosafety cabinet. Based on the biosafety cabinet, the invention further provides a corresponding device and a biological material taking method.