The invention relates to a right border flanking sequence of an exogenous insertion segment of genetically modified corn HiII-NGc-1, an application of the sequence, a primer pair, a reagent kit and ause method of the reagent kit. The right border flanking sequence can specifically indicate the genetically modified corn HiII-NGc-1; the primer pair, the reagent kit and the use method of the reagentkit are based on the right border flanking sequence; whether a sample to be detected is the genetically modified corn HiII-NGc-1 can be detected quickly and accurately; and the right border flankingsequence has important significance on improvement of identification efficiency of the genetically modified corn HiII-NGc-1, management and detection of a genetically modified product and identification of a germplasm resource.