The invention belongs to the technical field of winter rape breeding, and particularly relates to a strong-cold-resistance Chinese cabbage type winter rape temperature sensitive male sterile mutant identification and screening method. On the basis of a set of strictly specified culture management, the sterility screening and identification is performed in the specified growth period of different ecological regions; a specifically designed artificial climate culture box is used for processing; the obtained sterile male fertility result can accurately reflect the sterile sterility characteristics. The invention provides the key technology for the identification and screening method of the Chinese cabbage type winter rape temperature sensitive male sterile mutant in China. The advantages of operation simplicity, reliability, high work efficiency and the like can be realized. The method is applicable to different Chinese cabbage type winter rape temperature sensitive male sterile mutant identification and screening.