The invention discloses a preparation and an application for an electro-Fenton cathode material based on a loaded activated carbon fiber. The preparation comprises the following steps: generating hydrogen peroxide by dissolved oxygen on an activated carbon fiber cathode surface through the oxygen reduction reaction of two electrons; generating strong oxidizer free hydroxyl radical through the reaction of the generated hydrogen peroxide and the iron ion or copper ion complex catalyst loaded on the activated carbon fiber cathode surface; oxidizing and removing the refractory organic pollutants under a pH neutral condition. The activated carbon fiber Electro-Fenton cathode is simple in preparation method, the condition is mild, hydrogen peroxide is generated in-situ and the potential risk inthe transportation and storage processes of the activated carbon fiber Electro-Fenton cathode can be avoided; the treating process is clean, the Fe(II) agent need not be additionally and continuouslyadded, the sludge yield is reduced secondary pollution is not generated; according to the invention, the raw materials are low-cost and are easily acquired, the preparation method is simple, the treating period is short, the preparation method is easily combined with the other treating methods and the large-scale production and the comprehensive treatment for organic pollutants are benefited.