The invention discloses a layer-by-layer self-assembled silver nanowire flexible conductive film. A polymer film substrate is firstly modified by using polydopamine and then silver nanowires are formed through layer-by-layer self-assembly to obtain the flexible conductive film with excellent light transmission property, conductivity and adhesion property. The layer-by-layer self-assembled silver nanowire flexible conductive film has important application value in the field of flexible photoelectric devices, such as a display screen, a solar cell and a wearable device.