The invention discloses application of a Wuzi Yanzong pill, particularly application of the Wuzi Yanzong pill as a medicine for treating multiple sclerosis. The Wuzi Yanzong pill is used for intervening in EVE mice, the treatment function and the function mechanism of the Wuzi Yanzong pill are probed to provide experimental evidence for preventing and treating multiple sclerosis through clinical application of a kidney invigorating and essence replenishing method, and that the Wuzi Yanzong pill can serve as a medicine for treating multiple sclerosis is effectively testified.