The invention relates to a pleurotus ostreatus small molecule peptide compound wine and a preparation method thereof, and belongs to the technical field of liquid processing, which aims at providing anew wine for market. The pleurotus ostreatus small molecule peptide compound wine is characterized in that pleurotus ostreatus small molecule peptide powder is used as the main material, and has theeffects of dispelling wind and removing coldness, relaxing tendons and activating collaterals, treating waist and leg pain, numbness of hands and feet, and obstruction in channels and collaterals, inhibiting cancer cells, regulating hepatitis and chronic gastritis, stomach and duodenal ulcer, chondropathy, hypertension, urethral calculus and women climacteric syndrome, prolonging life, and promoting brain development; polysaccharides, vitamins, trace elements, essential oils and other materials are used as the auxiliary materials; the main material and the auxiliary materials are weighed, mixed according to different ratio, dried, and packaged, so as to obtain mixed peptide powder; the mixed peptide powder is mixed with primary pulp wine according to a ratio of 1:50, so as to obtain the peptide wine with nutrient supplementing effect.