The invention discloses an optimized soilless substrate for gerbera jamesonii bolus pot culture. The optimized soilless substrate is characterized by being composed of leaf mould and vermiculite, wherein the volume ratio of the leaf mould to the vermiculite is 3:1, the leaf mould does not contain litter of Eucalyptus spp. or Acacia dealbata Link., and the volume of the broad-leaf plant litter in the leaf mould accounts for 65-75% of the total volume of the leaf mould. Before the leaf mould is mixed with the vermiculite, the leaf mould is placed for six months or more, and the leaf mould is sieved through a sieve with a sieve hole diameter of 3cm. Compared with gerbera jamesonii bolus cultured by adopting traditional soil culture, the gerbera jamesonii bolus cultured by adopting the optimized soilless substrate provided by the invention blooms 5-10d in advance, the flowering branches are thick and strong, the leaf color is deeply green, the flowering branches are increased by 1-5cm, thediameter of the inflorescence is increased by 1-2cm, and the flowering period is prolonged by 2-4d, and the optimized soilless substrate is an effective means to alleviate continuous cropping obstacles and reduce infectious diseases; and meanwhile, the leaf mould is easy to obtain, the prepared mixed soilless substrate has a lower price, so that the production costs are saved, and the optimized soilless substrate has important significance to promoting of the sustainable development of the gerbera jamesonii bolus industries.