The invention discloses a pharmaceutical composition. The preparation is prepared from, by weight, 2-10 parts of polygonatum sibiricum, 1-5 parts of donkey-hide gelatin, 1-6 parts of polygonatum odoratum, 1-5 parts of lily flowers, 3-12 parts of rhizoma imperatae and 2-10 parts of rhizoma phragmitis. The invention further provides a preparation method and application of the pharmaceutical composition. The pharmaceutical composition is precise and appropriate in compatibility, all the medicine materials complement one another, insomnia, asthenic fever and drying, deficienc-fire dysphoria and restlessness and other symptoms caused by the constitution with Yin-deficiency can be significantly alleviated and treated, and the pharmaceutical composition can be used for effectively regulating theconstitution with Yin-deficiency and provides a new choice for clinical medication.