The invention discloses anti-static dust-collecting water-blocking cloth rubber shoe adhesive, and a preparation method and a use method thereof. The adhesion property of a rubber shoe foxing strip and polyester filament yarn fine grained cloth can be greatly improved through a unique adhesion system, which consists of an upper fiber water-blocking grafting agent, an adhesion transitional agent, an adhesive and a rubber surface treating agent, and under the specific use method. The adhesive has high adhesive strength and the cloth rubber shoe has waterproof and dustproof effects, so the wear-life of the rubber shoe is prolonged and the appearance quality of the rubber shoe is improved. The method provided by the method is simple and easy to industrialize.