The invention discloses a display substrate, a display panel, a substrate, a touch substrate and a substrate cutting method. The display substrate comprises a plurality of assumed touch electrodes anda plurality of connection wires, wherein the assumed touch electrodes are located in an assumed touch area; the connection wires are located in a binding area and connected with the assumed touch electrodes; the binding area is provided with a grounding electrode electrically connected with the plurality of connection wires; a first cutting alignment mark is arranged at the side, close to the connection wires, of the grounding electrode; the display panel comprises the display substrate; the substrate comprises the touch area, the binding area, the grounding electrode, the first cutting alignment mark and the second cutting alignment mark; the substrate cutting method comprises a method of cutting the substrate through the first cutting alignment mark and the second cutting alignment mark; the touch substrate is cut by the substrate cutting method; and the display substrate is cut from the substrate after a touch membrane of the substrate is scratched or corroded to lose effect, so that the cost is saved and the substrate material waste is avoided.