The invention relates to a manufacture method of a fluorescent screen for a transmission electron microscope. The fluorescent screen includes a substrate layer, a fluorescent powder layer and an aluminum film layer, wherein the substrate layer and the fluorescent powder layer are bonded with an inorganic binder. The method specifically includes the following steps that: a potassium silicate aqueous solution is prepared; an electrolyte is prepared, an appropriate amount of fluorescent powder is selected, so that a suspension is formed; a soluble barium salt or strontium salt aqueous solution isadded into the suspension, and an obtained mixed solution undergoes 30 minutes of still standing, and then, the mixed solution clarifies, a large part of the fluorescent powder deposits at the bottomof the mixed solution; the overflow valve of a sedimentation tank is opened, a fluorescent layer with a thickness of 10 to 30 micrometers can be formed; and the substrate which has been subjected toaluminum evaporation is arranged into a muffle furnace so as to be calcined at a high temperature of 500 DEG C for one hour, and the calcined substrate naturally cools, and then the cooled substrate is taken out. The aluminum film which is obtained through vacuum evaporation has good flatness and good reflectivity, and therefore, the brightness of the fluorescent screen is greatly improved. With the manufacture method of the invention adopted, the service life problem of the fluorescent screen can be solved in an emphasized manner, and the brightness and resolution rate of the fluorescent screen can be improved; and an aqueous dispersion system is used in a manufacture process, and therefore, organic pollution can be avoided.