The invention provides a preparation method of an enhanced environment-friendly diatom ooze coating. Activated lignin prepared through microbial fermentation and phenol react, phenol is partially replaced, hyperbranched polyester is taken as a medium, porous material quartz powder loaded negative ion powder and lignin replace phenol and formaldehyde and then are mixed with diatomite, hydroxyethylcellulose, inorganic pigments and calcium hydroxide at a high speed, and the coating is prepared. The prepared enhanced environment-friendly diatom ooze coating has high wear resistance and curing speed, releases very few harmful substances such as formaldehyde, can release negative oxygen ions when making contact with the air, has good air purification capability, belongs to an environment-friendly polymer material and has excellent development potentials.