The invention discloses a production method of a cell block paraffin section for fully presenting the position of a malignant cell. The method comprises the following steps: firstly, acquiring a primary cell sample and performing centrifugation sediment on the primary cell sample, so as to obtain a secondary cell sample; after the secondary cell sample is fixed, placing the secondary cell sample into a tissue dehydration box in a manner of vertical dividing from the middle for dehydration, transparentizing and waxing in sequence, so as to obtain a cell fixed sample; and vertically placing thecell fixed sampled into a tissue embedding box for embedding by taking a plane of section as a bottom surface, so as to form the section finally. Each section produced by the method can present the same cell hierarchical structure, so that the malignant cell appears at the same position of each section, a phenomenon that the malignant cell is located unreliably and even disappears in continuous sections is avoided, a comparative analysis when various antibodies are labeled in immunocytochemistry is facilitated, and cytopathology doctors can be effectively assisted to judge the source and the type of a malignant tumor, and therefore, the accuracy rate of cytopathological diagnosis is improved, a clinical application effect is good, and the practical value is high.