The invention provides a method for exercising and transplanting syringa reticulata tissue culture seedlings induced by callus tissues, and belongs to the technical field of plant tissue culture. Themethod for exercising and transplanting the syringa reticulata tissue culture seedlings induced by the callus tissues includes acquiring the syringa reticulata tissue culture seedlings induced by thecallus tissues and exercising the syringa reticulata tissue culture seedlings induced by the callus tissues for 5-10 d under the conditions of the seedling exercising temperatures of 15-18 DEG C, theillumination intensity of 800-100 lx, the daily illumination time of 5-8 h and the seedling exercising humidity of 70-80% so as to obtain exercised seedlings; transplanting the exercised seedlings intransplanting substrates and adding nutrient solution into the substrates at the roots of the seedlings on a daily basis. The method has the advantages that the transplanting survival rate of the syringa reticulata tissue culture seedlings induced by the callus tissues can reach 91% at least by the aid of procedures for exercising and transplanting the syringa reticulata tissue culture seedlings,the method is easy to implement and low in labor cost, and the problem of low survival rate of existing tissue culture seedlings induced by syringa reticulata callus tissues after the existing tissueculture seedlings are transplanted can be solved by the aid of the method.