The invention provides a glass micropipette electrode based on liquid metal and a preparation method of the glass micropipette electrode. The glass micropipette electrode comprises an ultra-fine glasstube (1), liquid metal (2), a packaging adhesive (3) and a connecting wire (4); the ultra-fine glass tube (1) is conical, the inner diameter of the tip of the ultra-fine glass tube is 1-5 micrometers, and the inner diameter of the other end of the ultra-fine glass tube is 1-2 mm; the liquid metal (2) is poured into the ultra-fine glass tube (1); the thick end of the ultra-fine glass tube (1) is filled with the packaging adhesive (3), the connecting wire (4) is inserted into the packaging adhesive (3), one end of the connecting wire (4) is in contact with the liquid metal, and the other end ofthe connecting wire (4) extends out of the ultra-fine glass tube (1) and is connected with a detection instrument. Compared with a traditional glass micropipette electrode, the glass micropipette electrode has a smoother electrode surface and lower electrical impedance characteristic, and is stable in electrochemical property. The preparation process is simpler, the cost is lower, and the controllability is better. Furthermore, a plurality of glass capillary tubes can be combined into a micropipette electrode array, so that physiological signal detection and electrical stimulation of a plurality of sites can be achieved.