The invention discloses a method for extracting and separating tetracycline antibiotics in milk powder by utilizing a metal organic framework-molecular imprinting compound material, and relates to a method for extracting and separating antibiotic residues in the milk powder. The invention aims to solve the problems that existing methods for detecting tetracycline antibiotics in milk powder have complex processes, poor selectivity and large organic solvent consumption. The method of the invention comprises taking the metal organic framework-molecular imprinting compound material as an adsorbent, taking Na2EDTA as a masking agent, mixing the adsorbent, the masking agent and milk powder to prepare an extraction column, taking an acetonitrile aqueous solution as a leaching liquid, conducting leaching, utilizing an acetic acid methanol solution as an eluant, and conducting elution so as to finish the extraction separation of tetracycline antibiotics in the milk powder. The method has the advantages that the adsorption amount of molecular imprinting is improved. The selection coefficient of a detection target analyte is 2.5 to 3.5. The method is mainly used for extracting and separatingtetracycline antibiotics in milk powder.