The invention relates to an adjusting device for outlet pressure of a microfluidic chip. The adjusting device is composed of two parts. A first part is a liquid storage system, wherein a pressure adjusting tube array is utilized for adjusting the liquid level height of a liquid storage tank for generating different liquid static pressures, and furthermore an additional static pressure is transferred to the outlet of the microfluidic chip through a pressure leading-out tube, thereby realizing a pressure adjusting operation. A second part is a supporting system, wherein a position fixing platform and a fastening bolt are used for relatively fixing the liquid storage system and the supporting system, thereby ensuring high stability of the system in operation. The adjusting device is characterized in that constant-pressure air pump pressure adjusting equipment realizes real-time adjustment on the outlet pressure of the microfluidic chip just according to a hydrostatic pressure principle onthe condition of no energy consumption and no noise. The adjusting device for the outlet pressure of the microfluidic chip has advantages of small size, high convenience in storage and transportation, low machining cost, and effective experiment threshold reduction. Furthermore the adjusting device facilitates popularization of microfluidic experiment researching.