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发明公开
CN107711822A 一种大鳞副泥鳅精子保存液、活化液和孵化方法 无效 - 驳回

一种大鳞副泥鳅精子保存液、活化液和孵化方法
摘要：
本发明公开了一种大鳞副泥鳅精子保存液、活化液和孵化方法，属于水产养殖的技术领域，其中，精子保存液的组分包括：0.5％-1.0％柠檬酸三钠、1.0％-3.0％葡萄糖、0.1％-0.3％碳酸氢钠、0.02％-0.04％氯化钾、0.01％-0.02％VC；活化液的组分包括：1-2％葡萄糖、0.35％氯化钠；孵化方法步骤包括：将利用精子保存液保存的精液用活化液激活，再与卵子结合，受精卵置于网片上静水孵化。与现有技术相比，本发明通过创新大鳞副泥鳅在特定精子保存液中的激活方式，可确保精子具有最大活力，提高鱼卵受精率和孵化率，指导育苗生产。
摘要（英）：

The invention discloses a preservation solution, an activation solution and a hatching method for Paramisgurnus dabryanus sperms, belonging to the technical field of aquaculture. The sperm preservation solution comprises the following components: 0.5% to 1.0% of trisodium citrate, 1.0% to 3.0% of glucose, 0.1% to 0.3% of sodium bicarbonate, 0.02% to 0.04% of potassium chloride and 0.01% to 0.02% of VC; the activation solution comprises the following components: 1 to 2% of glucose and 0.35% of sodium chloride; and the hatching method comprises the following steps: activating sperms preserved with the sperm preservation solution by using the activation solution, allowing the sperms to combine with eggs, placing fertilized eggs on a mesh, and carrying out hatching in static water. Compared with the prior art, the invention has the following advantages: through innovation of the activation manner of the Paramisgurnus dabryanus sperms in a specific sperm preservation solution, the maximum vitality of the sperms can be guaranteed; the fertilization rate and the hatching rate of fish eggs are improved; and the fry breeding production is guided.

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