The invention discloses a scene generation system and method for autonomous vehicle measurement. The system comprises a testing content generation module, a testing site library, a testing site layoutmodule, and a testing route generation module. The testing content generation module is configured to acquire testing demands and generating corresponding testing contents according to the testing demands; the testing site library is configured to store characteristic information of testing sites; the testing site layout module is configured to selecting the testing site matched with the testingcontents according to the characteristic information of the testing sites, and generating and outputting testing layout parameters of the testing site; the testing route generation module is configured to generate and output corresponding testing route data according to the testing contents and the testing site selected by the testing site layout module. According to the scene generation system and method for autonomous vehicle measurement, corresponding measurement scenes can be generated and provided for testing of autonomous vehicles according to actual testing demands.