The invention discloses a method for testing induction of tobacco products on vascular endothelial cell apoptosis. The method is characterized in that tobacco product solutions are prepared and then are subjected to centrifugation and purification of filtration membranes, poisoning and exposure on human vascular endothelial cells with appropriate density which are inoculated and are cultured overnight are carried out, then Annexin V-FITC and PI dyeing on cell suspensions prepared through digestive cells can be carried out, and finally, cells are detected by using a flow-type cytometer so as toquickly analyze a cell apoptosis condition. The method has the advantages of simpleness and convenience in operation, high sensitivity, reliability in results and the like, and can accurately detectan induction condition of tobacco products of different types on the human vascular endothelial cell apoptosis.