The invention discloses a cryopreserved serum analyzer for distinguishing ultraviolet Raman fluorescence spectrum based on time domain. The cryopreserved serum analyzer comprises a machine body and internal components assembled in the machine body; the internal components mainly comprise a raspberry pie, an ultraviolet Raman laser, an ultraviolet Raman probe, a spectrometer, a sample chamber, a direct current stabilized power supply, a touch screen, and accessories. The cryopreserved serum analyzer has the beneficial effects that a method of constant temperature and initial calibration in thesample chamber is adopted to improve uniformity of test environment and repeatability of a test. The test of a cryopreserved serum sample conducts time domain resolution sequence Raman fluorescence spectrum collection. Different species of serum in heating process, due to differences of viscosity, turbidity, absorption and other characteristics, have different time domain sequence ultraviolet Raman fluorescence spectrum characteristics, so that difference of different species of time domain spectroscopy characteristics extracted by the method is much larger than spectroscopy difference of theconventional one-time measurement, and further the recognition rate of different species of serum is effectively improved.