The invention belongs to the field of ant killing drugs, and particularly provides a high-efficiency low-toxicity ant killing bait and a preparation method thereof. The ant killing bait comprises an ant killing drug and a filling bait, wherein the ant killing drug comprises tebufenozide and abamectin. The filling bait comprises wheat flour, corn flour, butter, peanut oil and diatomite. The preparation method comprises: mixing the diatomite with butter and peanut oil, adding the corn flour and water to obtain a paste, adding the wheat flour and water to obtain dough, and baking the dough crisp;and dissolving tebufenozide and abamectin in acetone to obtain the ant killing drug to soak the filling bait, carrying out drying in an oven, carrying out pulverization, and carrying out sieving witha 10-18-mesh sieve. The ant killing bait provided by the invention is prepared from bioactive substances or substances from natural sources, which have specific effects on the growth and developmentof solenopsis invicta, is harmless to people, livestock and environment, and has an obvious effect of killing the solenopsis invicta.