The invention belongs to the technical field of antibiotic detection, and in particular relates to a penicillamine probe with a fluorescence-ultraviolet dual-signal mode as well as application of thepenicillamine probe. The probe is constructed based on the fluorescence-ultraviolet dual-signal mode; a construction method of the probe mainly comprises the following steps: (1) synthesizing blue fluorescent carbon quantum dots; (2) preparing a gold nanoparticle solution; (3) preparing the probe. The probe is prepared from carbon dots and gold nanoparticles, wherein the gold nanoparticles can quench fluorescent light. After a sample to be detected is added, the fluorescent light of the carbon dots recovers, the change of weak fluorescence to strong fluorescence occurs, and the colors of the gold nanoparticles gradually change to blue from red, so that the qualitative and quantitative detection of liquid to be tested can be realized. Compared with the prior art, the penicillamine probe hasgood selectivity, is visual in process, has a linear detection range of 0.4-1.8mu M, does not need complicated equipment, and is simple to operate.