The invention discloses an anesthesia box suitable for large-sized sturgeons. The anesthesia box comprises a box body of a cuboid structure formed by welding a stainless steel material, the right sideof the box body is of an opening structure, a sliding door clamping groove is formed in the right side of the box body, a sliding door is installed in the sliding door clamping groove in a sliding fit mode, and a handle is installed on the top of the sliding door. A plurality of partition plate clamping grooves are formed in the left side of the body, partition plates are installed in the partition plate clamping grooves in a sliding fit mode respectively, and handles are arranged on the tops of the partition plates respectively. By means of the anesthesia box, the problem that the anesthesiaoperation of the large-sized sturgeons is inconvenient is solved, and the anesthesia efficiency of the large-sized sturgeons is improved.