The invention relates to a cholera diagnosis microfluidic apparatus with a member used for driving a sample liquid and capable of being rapidly disassembled, and belongs to the field of analytical testing. According to the present invention, the use of inexpensive and easily-processed polydimethylsiloxane (PDMS) to produce the substrate of a microfluidic chip for diagnosing cholera has a series ofproblems; and the cholera diagnosis microfluidic apparatus is characterized in that the substrate selects the PDMS having an original ecological surface, a chain ring type electromagnetic fixture provided with a miniature ultrasonic wave transducer is sleeved and positioned at the position close to the sample liquid flow terminal of the microfluidic chip, ultrasonic waves induce the sample liquidflow to flow from the sample injection end of the chip to the terminal direction along the pipeline of the chip, the pipeline of the chip is filled with a micro-scale silica particle filler, and themicro-scale silica particle filler is used for supporting the inner wall of the pipeline, compensating the original hydrophobic pipeline with the set of hydrophilic micro-flow channels, and extrudingand covering the inner wall surface of the pipeline so as to reduce the opportunity of the contact between biological macromolecules and the inner wall surface.