A biosensor thin-layer electrode for rapidly detecting a myocardial infarction protein marker comprises a substrate; at least one strip-shaped thin-layer electrode is prepared on the substrate by a chemical coating or electrochemical electroplating process; at least one layer containing an antibody reagent layer which can interact with a myocardial infarction protein marker antigen so as to generate a detected electric signal is prepared on the thin-layer electrode; the antibody reagent layer is adhered to the surface of the thin-layer electrode through an inorganic gel layer; the upper surface of the thin-layer electrode is adhered to a nanoparticle-containing layer, and the nanoparticle-containing layer and the antibody reagent layer jointly form a laminated body; the nanoparticle-containing layer is prepared from a macromolecular compound which is inert in electrochemistry; the thin-layer electrode is one of a metal layer, a carbon electrode layer and a silver electrode layer; the thin-layer electrode has the characteristics of simple structure, convenience in manufacturing, lower cost, high sensitivity and the like.