The invention discloses a sperm collection cup for breeding egg chicken and belongs to the field of poultry breeding. The sperm collection cup comprises a second sperm camber movably connected with a first sperm chamber, wherein the bottom of the first sperm chamber is connected with the top end of the second sperm chamber, the first sperm chamber is of a double-layer structure, and an inner chamber is arranged in the first sperm chamber and spaced from the outer wall of the first sperm chamber; the inner chamber is communicated with the second sperm chamber, the bottom of the inner chamber tilts towards the middle, a protrusion is arranged in the middle of the bottom surface of the inner chamber, and the diameter of the bottom surface of the protrusion is equal to the diameter of a channel allowing the inner chamber to be communicated with the second sperm chamber. The sperm collection cup has the advantage that during collection, impurities which are collected accidentally can be filtered out, sperm can be diluted during collection, a proper sperm storage condition can be provided so as to guarantee the vigor of sperm, and then artificial insemination effect can be guaranteed.