利索-全球专利检索

  1. 登录
  2. 注册
发明公开
CN107793469A 一种去除宿主细胞蛋白含量的亲和纯化工艺 有权 转让

一种去除宿主细胞蛋白含量的亲和纯化工艺
摘要：
本发明提供一种新的有效降低宿主细胞蛋白(HCP)含量的亲和纯化工艺，其具体步骤包括：步骤1、处理亲和填料，上样；步骤2、淋洗，使用pH值较低的缓冲液(3.9～5.6)，以及使用含有氯化钠、氯化钙和/或精氨酸盐酸作为添加剂的淋洗缓冲液2淋洗层析柱；步骤3、洗脱，使用不同pH洗脱缓冲液洗脱产品并收集产品。本发明所使用的材料去除HCP效果显著，适用范围广，可广泛适用于抗体类蛋白质亲和纯化工艺中，从而为有效控制抗体类药物中HCP的含量提供技术支持。
摘要（英）：

The invention provides a novel affinity purification process capable of effectively lowering host cell protein (HCP) content. The affinity purification process specifically includes the steps of firstly, processing affinity filler, and performing sample loading; secondly, using a buffer solution with low pH (3.9-5.6) and an elution buffer solution 2 using sodium chloride, calcium chloride and/or arginine hydrochloric acid as additives to elute a chromatographic column; thirdly, using different pH elution buffer solutions to elute a product, and collecting the product. The affinity purificationprocess has the advantages that materials used by the affinity purification process are evident in HCP removing effect, wide in application range and widely applicable to the affinity purification process of antibody protein, and accordingly technical support is provided for the effective control of HCP content in antibody medicine.

公开/授权文献
0/0