The invention discloses an apparatus and a method for manufacturing single-cell fixation needle and belongs to the field of micromachining. The surface of a metal threaded rod which is coated with a coating is wound with a nichrome wire; the metal threaded rod wound with the nichrome wire is fixed to a quartz plate; one end of the nichrome wire wound on the metal threaded rod is fixed to the quartz plate and the other end thereof extends out of the edge of the quartz plate and then turned back to the quartz plate to form a nichrome wire ring tip at the suspension position of the edge of the quartz plate. Two ends of the nichrome wire are respectively connected with an output end of a voltage regulator through a conductor wire. A glass capillary with a tip end is fixed to a three-dimensional control table. By regulating the glass capillary with the tip end to the position close to the nichrome wire ring tip, and regulating the output voltage through a voltage regulator, heat output of the nichrome wire is controlled to achieve manufacturing of fixation needles having different inside and outside diameter ratio, so that fixation of cells with diameter ranging from several microns tohundreds of microns can be achieved.