The invention provides a method for preparing a graphene and nano-silver composite non-woven fabric. The method comprises the following steps that S1, polyolefin resin and/or polyester resin powder, graphene powder and nano-silver powder are mixed to be prepared into skin layer master batches; S2, polyolefin resin and/or polyester resin are/is prepared into core layer mater batches; S3, the skin layer master batches prepared in S1 and the core layer master batches prepared in S2 are prepared into the composite non-woven fabric through a non-woven fabric process. According to the method, graphene and nano-silver are mixed into graphene fiber raw materials, graphene and nano-silver are distributed in non-woven fabric fibers, and the very good antibacterial property is achieved.