The invention relates to the field of artificial synthetic leather, and discloses a preparation method of breathable and moisture-absorbent PU leather. The method comprises the following steps: (1) preparing primarily modified sepiolite; (2) preparing secondarily modified sepiolite; (3) preparing polyurethane resin; (4) preparing a wet base; (5) preparing a dry veneer; and (6) laminating the wet base with the dry veneer, successively drying and cooling, and then peeling release paper to prepare a finished product. According to the PU leather prepared by the method, the moisture absorption property and the breathability of the PU leather can be effectively enhanced; meanwhile, the wear resistance of the PU leather is effectively improved under the condition that the hand feeling of the PU leather is maintained.