The invention discloses a method for evaluating the biological toxicity and hereditary effect of a brominated flame retardant. After an organism is exposed to a brominated flame retardant, a sample pretreatment is carried out; the contents of dopa and lactic acid in the sample are detected by gas chromatography mass spectrometry (GC-MS); based on a binary logistic regression model, the probabilityvalue of classification forecasting on the sample is calculated with respective to the combined biological indexes; then according to a determined critical value, the biological toxicity and hereditary effect of the brominated flame retardant are evaluated, and the diagnostic performance of the brominated flame retardant is analyzed. The method has the characteristics of mild reaction conditions,simple operation, high repeatability, and high stability, can highly sensitively and specifically evaluate the biological toxicity and hereditary effect of a brominated flame retardant, and has a good application prospect.