The invention discloses an audio test system and method of a communication module. The audio test system comprises a test control module, a test service module and a test service control module; the test control module is used for transmitting target DTMF audio data to the communication module and the test service module in sequence, obtaining first recording data via the test service control module, transmitting the first recording data back to the test service module and the communication module in sequence and obtaining second recording data via the test control module; and a judging unit of the test control module is used for judging whether a DTMF code value of the second recording data is consistent with the DTMF code value of the target DTMF audio data, and determining whether an audio circuit of the communication module has a fault. According to the audio test system and method, no manual participation is needed, and whether the audio circuit of the communication module has thefault is automatically judged, thereby effectively improving the identification precision and the production efficiency.