The invention provides a large-scale production method of gypsophila paniculata L potted seedlings. The large-scale production method comprises the following steps: selecting newly germinated branch buds on a strong branch in December as explants, and carrying out induction, multiplication, seedling strengthening, rooting culture, ex vitro cutting and rooting maintenance, and potted seedling fieldplanting and maintenance, thus finally obtaining high-quality gypsophila paniculata L potted seedlings. The large-scale production method provided by the invention is characterized in that an LED (Light Emitting Diode) light recipe adopted during induction and multiplication stages comprises red light, blue light and white light which are 5 to 2 to 3 in ratio, and the LED light recipe adopted during seedling strengthening and rooting culture stages comprises red light, blue light, white light and far red light which are 5 to 1 to 2 to 2 in ratio; compared with a traditional fluorescent lamp,the electric energy is saved by 43.7 to 53 percent, and meanwhile, the multiplication rate and the seedling strengthening rate are respectively increased by 24.1 percent and 40 percent; rooting seedlings can be preserved at low cost for a long term only on the premise of water supply after the ex vitro cutting, flexible and sufficient supply of seed seedlings can be ensured, a high-quality gypsophila paniculata L potted seedling field planting and maintaining technology is established, and the large-scale production of the gypsophila paniculata L potted seedlings is realized.