The invention discloses a closed storage container of a urine sample. The closed storage container comprises a cup body, a handle, a urine discharging mechanism and a cup cover, wherein the cup body is used for collecting and containing urine; the handle is fixedly installed on the outer wall of the cup body and used for holding; the urine discharging mechanism is used for discharging the urine out of the cup body; the cup cover is used for sealing the cup body. The urine discharging mechanism comprises a urethral catheter and a urine outlet pipe, wherein the urethral catheter is arranged on the bottom of the cup body, and the urethral catheter and the cup body are integrally designed; the urine outlet pipe is coaxially matched with the urethral catheter and used for outputting the urine out of the inside of the cup body. According to the closed storage container, the urine discharging mechanism is added on the basis of a traditional urine cup, the cup cover does not need to be openedin the whole sampling process, and the problems that during sampling inspection, it is difficult to take urine, and the urine is contaminated are effectively solved.