The invention relates to the technical field of flue-cured tobacco seedlings, in particular to a flue-cured small strong seedling culture method. The method includes the following steps of sowing flue-cured seeds in a seedling bed to be cultured, adjusting the initial nitrogen concentration of a seedling bed nutrient solution to be 150-200 mg/kg when seedlings emerge, and spraying paclobutrazol onleaf surfaces in the culture process. In combination with other flue-cured floating seedling breeding technologies, the cultured flue-cured seedlings have a true green leaf color, the straw height of3-5 cm and the root dry weight larger than or equal to 0.04 gram per plant, and compared with a conventional seedling breeding mode at the same period, root vitality is improved by 4.85% or above, the net photosynthetic rate of a third visible leaf downward from the top is increased by 17.44% or above, the planting survival rate is increased by 3.16% or above, and the seedling recovery time is shortened by 48 h after transplanting.