The invention discloses a curcuma zedoary stem top vitrification ultralow temperature storage and unfreezing method, which mainly comprises the steps of cluster bud induced culture, subculture, pre-culture, loading liquid treatment, vitrification protection liquid treatment, fast freezing and unfreezing treatment and the like. The technical procedure of ultralow temperature storage of curcuma zedoary germplasm resources is built; the blank of the curcuma zedoary stem top ultralow temperature storage technology is made up. The used frozen protection agent has low cost and low toxicity; a freezing medium of liquid nitrogen has stable property, can be easily prepared and has low price; in addition, the operation of the ultralow temperature storage is simple; the mechanical damage on the stemtops in various operation processes is reduced; the viability of the stored stem tops can still be as high as 79 percent; the long-period stable storage can be realized after the once storage; the requirement of complicated operation of frequent subculture of conventional planting and tissue culture seedling is simplified. An unfreezing method provided by the invention has the advantages that theoperation is simple; in the cell unfreezing process, the mechanical damage does not occur; the viability of the curcuma zedoary stem tops is not influenced.