The invention discloses a mineral beneficiation method of complex and refractory high-grade copper sulfide ore. The method comprises the steps that high-grade copper sulfide raw ore is crushed; the ore is ground; pulp is adjusted; high-grade copper sulfide ore rough scavenging is performed; and concentrate roughing, grinding and selection are performed in sequence. According to the mineral beneficiation method of the complex and refractory high-grade copper sulfide ore, through an efficient inhibitor XKY-03, inhibition of pyrite minerals is enhanced selectively, and inevitable activation of ions to the pyrite minerals in the ore pulp is eliminated selectively; through an efficient collector XKP-03, collecting of copper sulfide minerals is selectively enhanced; and meanwhile, before flotation, by adopting quick lime and NaOH, the pH of the ore pulp is adjusted to be strongly alkaline, then flotation is performed, part of the pyrite minerals is inhibited, influences of the pyrite minerals on flotation of the copper sulfide minerals are reduced, and accordingly, the copper sulfide minerals, the pyrite minerals and gangue minerals are efficiently separated. The mineral beneficiation method has the characteristics of being good in separation effect, stable in mineral beneficiation index, small in agent consumption amount, easy and convenient to operate and easy to operate and manageon site, is a novel method suitable for mineral beneficiation of the complex and refractory high-grade copper sulfide ore, and is suitable for application and popularization.