The invention belongs to the technical field of fuel, and particularly relates to vehicle biofuel. The vehicle biofuel is prepared from the following raw materials in parts by weight: 60 to 80 parts of biodiesel, 8 to 15 parts of 2, 2-isoamyl ether-1, 3-propylene glycol, 8 to 12 parts of propanediol butyl ether, 3 to 8 parts of triallylamine, 1 to 5 parts of glycerin monostearate, and 1 to 5 partsof dimethylacetamide. The vehicle biofuel has a self-cleaning effect, is unlikely to cause carbon deposition, has a relatively long guarantee period, is unlikely to go bad, and ensures that biofuel prepared from the vehicle biofuel meets the technical requirements of national standard V.