The invention discloses a photosensitive resin composition, a pixel defining layer using the same, and a display device. The photosensitive resin composition includes (A) a binder resin; (B) a photopolymerizable monomer; (C) a photopolymerization initiator including a first photopolymerization initiator and a second photopolymerization initiator; (D) a black colorant; and (E) a solvent, wherein the first photopolymerization initiator is represented by Chemical Formula 1, the second photopolymerization initiator has a different maximum absorption wavelength from the first photopolymerization initiator, and the first photopolymerization initiator and the second photopolymerization initiator are included in a weight ratio of about 3:7 to about 7:3. In the Chemical Formula 1 as shown in the description, each substituent is the same as the one defined in the specification.