The invention discloses a positioning structure for transmission and winding of cigarette paper. The positioning structure comprises a collecting reel, a positioning reel and a storage reel; the collecting reel and the positioning reel are located on the same horizontal plane and are fixedly connected with each other through two symmetric limiting baffles; the cigarette paper is fixed on the storage reel, passes through the bottom of the positioning reel and the gap between the limiting baffles sequentially and is connected onto the collecting reel; the outsides of bolts at two ends of the collecting reel, at two ends of the positioning reel and at two ends of the storage reel are sleeved with limiting gears; and connecting blocks perpendicularly welded on third supporting rods are furtherarranged at the ends, connected with the storage reel, of the third supporting rods. The limiting gears at the two ends of the storage reel abut against retarding stop rods on the connecting blocks,so that the rotating speed of the storage reel can be limited; and secondly, the limiting baffles capable of limiting the position of the cigarette paper are arranged between the collecting reel and the positioning reel, so that the cigarette paper is prevented from deviating from the position when collected, and the collecting effect on the cigarette paper is better.