The invention provides a synthetic rubber material-magnetic nanoparticle based gecko biomimetic surface, preparation and application. The biomimetic surface is in a gecko seta shape and contains a fiber array, wherein each fiber of the fiber array comprises a seta spindle and listric vibrissae at the tip of the seta spindle. In terms of total weight 100% of raw materials for preparing the gecko biomimetic surface, the gecko biomimetic surface is prepared from a synthetic rubber material, a curing agent and 1wt%-50wt% of magnetic nanoparticles, and the mass ratio of the curing agent to the synthetic rubber material is (1-100):1. Different types of magnetic nanoparticles with different particle sizes and content are added to the synthetic rubber material before the synthetic rubber materialis poured to a template for curing, so that the Young modulus and other physicochemical properties such as magnetism, hardness, wettability and adhesion of the gecko biomimetic surface can be adjusted, and the response performance of the gecko biomimetic surface can be improved substantially.