The invention discloses a novel biochemical preparation method for macroporous white carbon black. The method adopts water glass as a precursor, biological starch as a template agent, an inorganic acid as a catalyst, water as a solvent, and a very small amount of amylase as a template removing agent. In the preparation process of the macroporous white carbon black provided by the invention, the biological starch is used as the template agent, so that the pore size of the white carbon black is effectively increased, the technological process is simple, green and environmentally friendly, and the production cycle is short; in addition, the production raw materials are cheap, the production costs are low, and the yield is high; and a small amount of the biological enzyme is added during the preparation of the macroporous white carbon black, so that the template agent starch is highly efficiently and mildly degraded, and the collapse and damage of nano pore channels can be effectively prevented, thereby ensuring the product quality of the macroporous white carbon black.