The invention belongs to the technical field of cultivation of sugarcanes and specifically provides a method of industrialized production of single bud seed stems of the sugarcanes and application ofthe method. The method comprises the following steps: selecting improved varieties of sugarcanes and second-generation seed stems of virus-free healthy seedlings or virus-free seedlings, planting theimproved varieties of sugarcanes and the second-generation seed stems of the virus-free healthy seedlings or the virus-free seedlings in May or June and spraying a growth preparation at an elongationstage of the seedlings; harvesting sugarcane stems of healthy seedlings and cutting the sugarcane stems into single bud section sugarcane seeds with the length of 4cm with a seed cutting machine; performing special seed soaking coating on the single bud section sugarcane seeds; sub-packaging the single bud section sugarcane seeds with printed vacuum package plastic bags and sealing the plastic bags with a vacuum package machine; spraying two-dimensional codes on the package bags of products and gluing marks of quality conformance. The single bud seed stems of the sugarcanes, which are producedby the method, are high in quality, the germination rate and the tillering rate are above 96%, and the effects of disease prevention and insect prevention are good; the sowing quantity of new-planting sugarcanes can be reduced by above 200% (by weight), and the seed cost can be reduced by 30% or more; the method adapts to a mechanical matched planting technology and is high in efficacy.