The invention provides a method for preparing chloropropylene oxide from biomass glycerol. The method comprises the following steps: first mixing biomass glycerol with organic acid, carrying out heating to 90-140 DEG C, maintaining the temperature, introducing hydrogen chloride gas into the obtained system and starting a bubbling reaction, wherein tail gas is absorbed with alkali liquor; cooling amaterial obtained in the previous step to room temperature, then transferring the material to another reactor, heating the material to 30-90 DEG C, and adding a KOH solution with a concentration of 6-10 mol/L for a reaction; and at the end of the reaction, removing solids through filtration so as to obtain a filtrate which is a chloropropylene oxide solution. The method of the invention has the advantages of simple process, easy availability of raw materials, low cost and good industrialization prospects.