The invention relates to the technical field of medicines, in particular to a novel 4-methylene steroids compound. A chemical structure of the novel 4-methylene steroids compound is as shown in formula (I). The invention further provides an extracting method of the novel compound and anti-tumor application. The preparation method of the compound is simple, and anti-tumor activity is remarkable. Anew lead compound is provided for research and development of new anti-tumor drug, and a scientific basis is provided for development and utilization of marine medical resources in China.